Langfang DingYang

What Are The Factors Affecting The Performance of Large Flanges

fittingflange | 07 December, 2018 16:27

In the production of large flanges, there are many factors affecting the performance of large flanges. Let's talk about the following common factors: first, annealing temperature (annealing temperature is the temperature parameter when primers and templates are combined, and when 50% of primers and complementary sequences are double-stranded DNA molecules, it is the temperature that affects the specificity of PCR. Factors. Ideally, the annealing temperature is low enough to ensure the effective annealing of primers with the same target sequence, and high enough to reduce non-specific binding. Reasonable annealing temperature ranges from 55 to 70 C. The annealing temperature is generally set to be 5 C lower than the Tm of the primer. Whether the required temperature is reached or not. Large stainless steel flange is usually treated by solid solution heat treatment, which is commonly known as "annealing", the temperature range is 1040 ~ 1120 ("Japanese standard"). You can also observe through the annealing furnace observation hole that the large flanged pipes in the annealing zone should be incandescent, but there is no softening sag. Second, the sealability of the furnace body (the shell is welded by steel plate and section steel, the trolley is welded by section steel and steel plate, and the trolley reduces the heat radiation and convection loss by soft contact with the lining and sand sealing mechanism, thus effectively ensuring the sealability of the furnace body). Bright annealing furnace should be closed, isolated from the outside air; hydrogen as protective gas, only one exhaust port is open (used to ignite the exhaust hydrogen). Soap water can be used to smear the cracks in each joint of the annealing furnace to see if it runs out. The most easily runs out is the place where the annealing furnace enters and leaves the tube. The sealing rings in this place are particularly easy to wear and tear, so it is necessary to check and replace them frequently. Third, the protective pressure. In order to prevent micro-leakage, the protective gas in the furnace should maintain a certain positive pressure. If it is hydrogen protective gas, the general requirement is more than 20 kBar. Annealing atmosphere: Pure hydrogen is generally used as annealing atmosphere. The purity of the atmosphere is better than 99.99%. If the other part of the atmosphere is inert gas, the purity can also be lower, but absolutely can not contain excessive oxygen and water vapor.


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